Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the applications of the protocols is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
Techniques explained in this reference include:
— Nucleic Acid (DNA/RNA) isolation
— Next-generation sequencing through real time PCR
— Western Blotting
— 2-D gel electrophoresis
— Chromatin immunoprecipitation (ChIP)
— Live cell-culture techniques
— Golgi Staining
— Recent laboratory protocols
— Diverse examples of molecular biology experiments
— Simple step-by-step presentation of information
— Special focus on scientific and clinical applications
Protocols used in Molecular Biology is essential reading for academicians, molecular biologists, as well as graduate and undergraduate students studying basic and applied research. Pharmacologists, and medical researchers can also benefit from the wide array of techniques presented in the book.